There are two different types of wrinkles on the face: static wrinkles and dynamic wrinkles. Static wrinkles are present even with complete stillness and lack of expression on the face. Dynamic wrinkles are those that appear or become significantly more apparent with facial expression. Botox is ideal for treatment of dynamic wrinkles of the face and is used to relax those facial muscles whose excessive action causes wrinkling of the skin. For example, the deep lines between the eyebrows (frown lines) are the most popular treatment sites.

Other areas which can be improved with Botox are the crow's feet and the horizontal forehead lines. Depending on the doctors' skill and experience, neckbands, certain lip lines, smile lines along the side of the nose, and droopy corners of the mouth may also be improved with Botox.